Title
Effect of Crotalus basiliscus snake venom on the redox reaction of myoglobin
Author
Ticyano P. de Souza, Dieric S. Abreu, Marta S. P. Carepo, Maria A. S. Silva, Dávila Zampieri, Marcos N. Eberlin, Tércio F. Paulo, Eduardo H. S. Sousa, Elisane Longhinotti, Izaura C. N. Diógenes
Year
2019
Journal
Journal of Biological Inorganic Chemistry
Abstract
In this work, we have studied the effect of Crotalus basiliscus snake venom on the redox reaction of myoglobin (Mb), and by means of electrochemical techniques, we have shown that this reaction is undoubtedly affected following the interaction with the venom. Surface plasmon resonance, electrophoresis, UV–Vis, and circular dichroism showed that the interaction involves the attachment of some constituent of the venom to the protein, although not affecting its first and secondary structures. Mass spectra support this suggestion by showing the appearance of signals assigned to the Mb dimer and to a new species resulting from the interaction between Mb and the venom proteins. In addition, the mass spectra suggest the aromatic amino acids of myoglobin, mainly tryptophan and phenylalanine, are more exposed to the solvent medium upon the exposure to the venom solution. The results altogether indicate that the harmful effects of the venom of Crotalus basiliscus snake are likely connected to the blocking of the redox site of Mb.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Tertiary structure, Soret region, Protein folding, Chemical stability, Biochemistry