Title
Interaction studies of haemoglobin with p-sulfonatocalix[8]arene by spectrophotometric methods
Author
Vijay Karbhari Ahire, Dipalee Dileep Malkhede
Year
2019
Journal
Chemical Physics Letters
Abstract
Interaction studies of haemoglobin (Hb) with p-sulfonatocalix[8]arene (p-SCX8) has been analysed through UV–visible, fluorescence and circular dichroism spectroscopy. The Ch-π, π-π stacking, van der Waals forces, electrostatic interaction, hydrogen bonding, hydrophobic and hydrophilic interactions may involve in protein-calixarene complexes. Binding constant, quenching constant, binding sites have been calculated from fluorescence data. The binding constant estimated on the order of 104 M−1. The key results can obtained from circular dichroism technique shows gradual decrease in alpha (α) helix concentration from 47.8 to 40.23%. It may be because of p-SCX8 being able to provide surface for the Hb molecule.
Instrument
J-1500
Keywords
Circular dichroism, Secondary structure, Ligand binding, Biochemistry