Title
Covalent versus Noncovalent Binding of Ruthenium η6‐p‐Cymene Complexes to Zinc‐Finger Protein NCp7
Author
Yaping Sheng, Zhuanghao Hou, Shiyong Cui, Kaiming Cao, Siming Yuan, Mei Sun, Jakob Kljun, Guangming Huang, Iztok Turel, Yangzhong Liu
Year
2019
Journal
Chemistry A European Journal
Abstract
Ruthenium–arene complexes are a unique class of organometallic compounds that have been shown to have prominent therapeutic potencies. Here, we have investigated the interactions of Ru‐cymene complexes with a zinc‐finger protein NCp7, aiming to understand the effects of various ligands on the reaction. Five different binding modes were observed on selected Ru‐complexes. Ru‐cymene complex can bind to proteins through either noncovalent binding alone or through a combination of covalent and noncovalent binding modes. Moreover, the noncovalent interaction can promote the coordination of RuII to NCp7, resulting synergistic effects of the different ligands. The binding of Ru(Cym) complexes leads to dysfunction of NCp7 through zinc‐ejection and structural perturbation. These results indicate that the reactivity of Ru‐complexes can be modulated by ligands through different approaches, which could be closely correlated to their different therapeutic effects.
Instrument
J-810
Keywords
Circular dichriosm, Protein folding, Secondary structure, Ligand binding, Coordination chemistry, Biochemistry, Inorganic chemistry