Title
A novel adaptive fluorescent probe for cell labelling
Author
Anca G. Coman, Anca Paun, Codruta C. Popescu, Niculina D. Hădade, Anamaria Hanganu, Gabriela Chiritoiu, Ileana C. Farcasanu, Mihaela Matache
Year
2019
Journal
Bioorganic Chemistry
Abstract
In this study we describe the synthesis and characterisation of a new hydrazone-based fluorescent compound that is able to selectively label the endoplasmic reticulum (ER) in yeast and mammalian living cells. The fluorescence properties of the compound depended on the DMSO/water ratio and on the pH. NMR experiments allowed determination of the conformation adopted in various environments. Apart from the convenient synthetic procedure, our compound displays low cell toxicity and blue emission compatible with filters routinely used in fluorescence microscopy.
Instrument
V-630, FP-8300
Keywords
Absorption, Chemical stability, Photophysical properties, Fluorescence, Biochemistry, Organic chemistry, Materials, Sensors