Title
Reconstituted Fibril from Heterogenic Collagens-A New Method to Regulate Properties of Collagen Gels
Author
Jian Yang, Haibo Wang, Lang He, Benmei Wei, Chengzhi Xu, Yuling Xu, Juntao Zhang, Sheng Li
Year
2019
Journal
Macromolecular Research
Abstract
Heterotypic collagen fibril has long been found in the tissues of organisms, which plays an important role in the formation and function of complex structures of organisms. Inspiring by the phenomenon, scholars tried to incubate collagens from different sources into novel collagen materials in vitro, and the forming of heterogenic reconstituted collagen fibrils (RF) was often demonstrated by differential scanning calorimetry (DSC) and fluorescence quenching analysis. In this work, we used two type-I collagens from different species (bovine tendon and grasscarp fish skin) to co-assemble in vitro, and verified the formation of RF from a new rheological perspective. In addition, we also investigated the assembly behavior, surface hydrophilicity and hydrophobicity, microscopic morphology and cell proliferation ability of the RF. The results showed that the assembly rate, surface properties, fibril size, viscoelastic properties of RF can be delicately regulated by the method of heterogenic collagen reconstitution. This study provides new experimental evidence for the reconstitution of heterogenic collagens, and also offers a new means for the regulation of collagen gel performance, which would help to expand the application range of collagen gel materials.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Collagen, Biochemistry