Title
Spiropyran labeling for sensitive probing of protein diffusion by the transient grating method
Author
Shunki Takaramoto, Yusuke Nakasone, Kei Sadakane, Shinsaku Maruta, Masahide Terazima
Year
2019
Journal
Chemical Physics Letters
Abstract
The pulsed laser induced transient grating method is a powerful technique for measuring the diffusion coefficient (D) sensitively with a high time-resolution. For expanding the applicability of this method, a labeling method with a spiropyran derivate is demonstrated. The signals upon photoexcitation of the labeled proteins were strong and it was simple to determine D accurately. D and the circular dichroism spectra at various concentrations of a denaturant were measured and it was found that D changes before the circular dichroism spectrum change at a low concentration region, indicating that D is a sensitive probe for monitoring protein conformation.
Instrument
J-720
Keywords
Circular dichroism, Secondary structure, Biochemistry