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Home / Applications / Analysis of Additives in Soft Drinks using a UHPLC System (X-LC)

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Analysis of Additives in Soft Drinks using a UHPLC System (X-LC)

By Miyuki Kanno

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January 8, 2024

Introduction

Food additives are substances added to food as preservatives, antioxidants, emulsifiers, etc. Recent increase in consumption of processed foods has introduced more additives. Securing food safety demands determination of these additives. High performance liquid chromatography (HPLC) has been used for the measurement of these food additives.

We examined the usefulness of an X-PressPak V-C (2.0 mm I.D. x 50 mm L.) packed with 2 µm diameter packing material for the ultra-high speed separation of food additives. We used a 4λUV detector for the simultaneous monitoring of these additives at four different wavelengths. The results were examined to determine whether the performance of the column and chromatography system exceeds those of conventional HPLC.

Experimental

Jasco XLC system for analysis of soft drinks additives

The system utilized in this experiment was a JASCO X-LC system consisting of two 3085-PU pumps, 3080-DG mobile phase degasser, 3080-MX mixing unit, 3067-CO column oven, 3177-UV 4λ-UV detector, 3059-AS autosampler, and ChromNAV chromatography data system.

The method was applied to the analysis of soft drinks. The soft drink was purchased at a grocery store, diluted 5 times with a mixture of 0.3% phosphoric acid /acetonitrile (95/5), and then filtered with 0.2 µm membrane filter.

Keywords

620017X

Results

Figure 1 shows an X-LC chromatogram of a standard mixture of 11 food additives. The X-LC system provides 5 times shorter analysis time than conventional HPLC without sacrificing the resolution.

Figure 1. X-LC chromatogram of a standard mixture of food additives.
Peaks: 1=acesulfame K (0.2 mg/mL), 2=p-hydroxybenzoic acid (0.05 mg/mL), 3=caffeine (0.05 mg/mL), 4=aspartame (0.1 mg/mL), 5=vitamin B2 (0.1 mg/mL), 6=benzoic acid (0.05 mg/mL), 7=methylparaben (0.05 mg/mL), 8=propyl gallate (0.05 mg/mL), 9=ethylparaben (0.05 mg/mL), 10=propylparaben (0.05 mg/mL), 11=butylparaben (0.05 mg) Chromatographic conditions: column=X-PressPak V-C18 (2.0 mmID x 50 mmL, 2 µm); column temperature=40°C; mobile phase: A=0.3% phosphoric acid, B=acetonitrile, 0min=A/B(95/5), 1.3min=A/B(85/15), 1.7min=A/B(85/15), 5min=A/B(30/70), 5.1 min=(10/90), 6 min=(10/90), 6.1 min=(95/5), injection interval=8 min; flow rate=0.5 mL/min; detection wavelengths=210 nm, 240 nm, 270 nm, 300 nm, injection volume=1 µL.
Figure 2. X-LC chromatogram of the soft drink.
The sample is diluted 5 times with 0.3% phosphoric acid/acetonitrile(95/5), and filtered with 0.2 µm membrane filter.
The other conditions are the same as in the Figure 1 caption.
This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

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About the Author

Miyuki Kanno

JASCO Application Note

Analysis of Additives in Soft Drinks using a UHPLC System (X-LC)

Introduction

Food additives are substances added to food as preservatives, antioxidants, emulsifiers, etc. Recent increase in consumption of processed foods has introduced more additives. Securing food safety demands determination of these additives. High performance liquid chromatography (HPLC) has been used for the measurement of these food additives.

We examined the usefulness of an X-PressPak V-C (2.0 mm I.D. x 50 mm L.) packed with 2 µm diameter packing material for the ultra-high speed separation of food additives. We used a 4λUV detector for the simultaneous monitoring of these additives at four different wavelengths. The results were examined to determine whether the performance of the column and chromatography system exceeds those of conventional HPLC.

Experimental

Jasco XLC system for analysis of soft drinks additives

The system utilized in this experiment was a JASCO X-LC system consisting of two 3085-PU pumps, 3080-DG mobile phase degasser, 3080-MX mixing unit, 3067-CO column oven, 3177-UV 4λ-UV detector, 3059-AS autosampler, and ChromNAV chromatography data system.

The method was applied to the analysis of soft drinks. The soft drink was purchased at a grocery store, diluted 5 times with a mixture of 0.3% phosphoric acid /acetonitrile (95/5), and then filtered with 0.2 µm membrane filter.

Results

Figure 1 shows an X-LC chromatogram of a standard mixture of 11 food additives. The X-LC system provides 5 times shorter analysis time than conventional HPLC without sacrificing the resolution.

Figure 1. X-LC chromatogram of a standard mixture of food additives.
Peaks: 1=acesulfame K (0.2 mg/mL), 2=p-hydroxybenzoic acid (0.05 mg/mL), 3=caffeine (0.05 mg/mL), 4=aspartame (0.1 mg/mL), 5=vitamin B2 (0.1 mg/mL), 6=benzoic acid (0.05 mg/mL), 7=methylparaben (0.05 mg/mL), 8=propyl gallate (0.05 mg/mL), 9=ethylparaben (0.05 mg/mL), 10=propylparaben (0.05 mg/mL), 11=butylparaben (0.05 mg) Chromatographic conditions: column=X-PressPak V-C18 (2.0 mmID x 50 mmL, 2 µm); column temperature=40°C; mobile phase: A=0.3% phosphoric acid, B=acetonitrile, 0min=A/B(95/5), 1.3min=A/B(85/15), 1.7min=A/B(85/15), 5min=A/B(30/70), 5.1 min=(10/90), 6 min=(10/90), 6.1 min=(95/5), injection interval=8 min; flow rate=0.5 mL/min; detection wavelengths=210 nm, 240 nm, 270 nm, 300 nm, injection volume=1 µL.
Figure 2. X-LC chromatogram of the soft drink.
The sample is diluted 5 times with 0.3% phosphoric acid/acetonitrile(95/5), and filtered with 0.2 µm membrane filter.
The other conditions are the same as in the Figure 1 caption.

Keywords

620017X

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.
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