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Home / Applications / Analysis of Fatty Acids using the LC-4000 Series Rapid Separation HPLC

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Analysis of Fatty Acids using the LC-4000 Series Rapid Separation HPLC

By Kenichi Akao

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October 9, 2024

Introduction

An analytical HPLC system using microparticulate packing materials requires a lower system volume, higher operating pressure and high speed response. Rapid separation HPLC (RHPLC) is likely to become the new standard for high performance analysis, covering measurement in a wide range of applications from conventional HPLC to some types of UHPLC. The following is an analysis report for the measurement of fatty acids using a Unifinepak C18 column with the RHPLC system including a refractive index detector (RI-4035) for a semi-micro scale measurement.

Experimental

EquipmentConditions
Pump:PU-4185Column:A) Unifinepak C18 (4.6 mmI.D. x 150 mmL, 5 μm)
Pump option:Degasser unit B) Unifinepak C18 (2.0 mmI.D. x 150 mmL, 1.9 μm)
Autosampler:AS-4150 C) Unifinepak C18 (2.0 mmI.D. x 30 mmL, 1.9 μm)
Column oven:CO-4060Eluent :0.1%TFA/Acetonitrile (20/80)
Detector:RI-4035Flow rate:A; 1.2 mL/min, B-C; 0.6 mL/min
  Column temp.:40ºC
  Injection volume:A; 10 μL, B-C; 2 μL
  Standard Sample:Fatty acids mixture (1 mg/mL per component)

Keywords

220058H

Results

Fig. 1 Chromatograms of fatty acids were measured using the LC-4000 series RHPLC system with conventional HPLC and sub 2 μm UHPLC columns. The RI-4035 refractive index detector is optimized for lower flow rates and allows the acquisition of chromatograms with improved peak shape and a higher resolution separation. Unifinepak offers a common packing material in a range of particle sizes, which is independent of the measurement method, such as column size.

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

Featured Products:

  • LC-4500 Series Compact HPLC

  • LC-4000 Series UHPLC

  • Single Quadrupole LC-MS

About the Author

Mr. Akao, is the Applications Group Manager at JASCO Corporation, with many years at the company working on applications in electronic and vibrational spectroscopy, and is an expert in IR and Raman spectroscopy.

JASCO Application Note

Analysis of Fatty Acids using the LC-4000 Series Rapid Separation HPLC

Introduction

An analytical HPLC system using microparticulate packing materials requires a lower system volume, higher operating pressure and high speed response. Rapid separation HPLC (RHPLC) is likely to become the new standard for high performance analysis, covering measurement in a wide range of applications from conventional HPLC to some types of UHPLC. The following is an analysis report for the measurement of fatty acids using a Unifinepak C18 column with the RHPLC system including a refractive index detector (RI-4035) for a semi-micro scale measurement.

Experimental

EquipmentConditions
Pump:PU-4185Column:A) Unifinepak C18 (4.6 mmI.D. x 150 mmL, 5 μm)
Pump option:Degasser unit B) Unifinepak C18 (2.0 mmI.D. x 150 mmL, 1.9 μm)
Autosampler:AS-4150 C) Unifinepak C18 (2.0 mmI.D. x 30 mmL, 1.9 μm)
Column oven:CO-4060Eluent :0.1%TFA/Acetonitrile (20/80)
Detector:RI-4035Flow rate:A; 1.2 mL/min, B-C; 0.6 mL/min
  Column temp.:40ºC
  Injection volume:A; 10 μL, B-C; 2 μL
  Standard Sample:Fatty acids mixture (1 mg/mL per component)

Results

Fig. 1 Chromatograms of fatty acids were measured using the LC-4000 series RHPLC system with conventional HPLC and sub 2 μm UHPLC columns. The RI-4035 refractive index detector is optimized for lower flow rates and allows the acquisition of chromatograms with improved peak shape and a higher resolution separation. Unifinepak offers a common packing material in a range of particle sizes, which is independent of the measurement method, such as column size.

Keywords

220058H

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.
28600 Mary’s Court, Easton, MD 21601 USA • (800) 333-5272 • Fax: (410) 822-7526 • jascoinc.com/applications

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