An analytical HPLC system using microparticulate packing materials requires a lower system volume, higher operating pressure and high speed response. Rapid separation HPLC (RHPLC) is likely to become the new standard for high performance analysis, covering measurement in a wide range of applications from conventional HPLC to some types of UHPLC. The following is an analysis report for the measurement of fatty acids using a Unifinepak C18 column with the RHPLC system including a refractive index detector (RI-4035) for a semi-micro scale measurement.
|Pump:||PU-4185||Column:||A) Unifinepak C18 (4.6 mmI.D. x 150 mmL, 5 μm)|
|Pump option:||Degasser unit||B) Unifinepak C18 (2.0 mmI.D. x 150 mmL, 1.9 μm)|
|Autosampler:||AS-4150||C) Unifinepak C18 (2.0 mmI.D. x 30 mmL, 1.9 μm)|
|Column oven:||CO-4060||Eluent :||0.1%TFA/Acetonitrile (20/80)|
|Detector:||RI-4035||Flow rate:||A; 1.2 mL/min, B-C; 0.6 mL/min|
|Injection volume:||A; 10 μL, B-C; 2 μL|
|Standard Sample:||Fatty acids mixture (1 mg/mL per component)|
Fig. 1 Chromatograms of fatty acids were measured using the LC-4000 series RHPLC system with conventional HPLC and sub 2 μm UHPLC columns. The RI-4035 refractive index detector is optimized for lower flow rates and allows the acquisition of chromatograms with improved peak shape and a higher resolution separation. Unifinepak offers a common packing material in a range of particle sizes, which is independent of the measurement method, such as column size.