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Home / Applications / Analysis of Nonionic Surfactants by HPLC-ELSD

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Analysis of Nonionic Surfactants by HPLC-ELSD

By Keisuke Watanabe

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August 19, 2022

Introduction

Surfactants are well known as a main constituent of commercial detergent and is widely used in various industrial and pharmaceutical fields due to various capabilities such as washing performance, moisture absorbency, osmosis, solubility, dispersibility, lubricity, antistatic ability, bactericidal property and anti-rust property. Nonionic surfactants have hydrophilic groups which are not ionized even when dissolved in water. Since it is hardly affected by water hardness and electrolytes, it can be used together with all other surfactants. Considering its nature, the usage of nonionic surfactants have increased dramatically and its affect on the environment has been pointed out. Since 2003, nonionic surfactants have been the test item for Water Quality Control Standards. This time, Triton X-100 and Polyethylene Glycol 400 as nonionic surfactant samples were measured and analyzed using an ELSD detector with Silica NH2 column in HILIC mode.

Experimental

Results

Figure 1 and figure 2 show the chromatograms of Triton X-100 and PEG400, respectively.

Figure 1 Chromatogram of Triton X-100.
Figure 2 Chromatogram of PEG400.
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About the Author

Keisuke Watanabe

JASCO provides services and support to a diverse range of industries throughout the world.

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