Analysis of Triglycerides by High Performance Liquid Chromatography with Evaporative Light Scattering Detection

By DJ Tognarelli

Download PDF August 19, 2022

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Introduction

Although triglycerides function as an energy source, it is recognized that too much intake may cause arteriosclerosis. Since most of components in triglycerides have almost no UV absorption, a differential refractive index detector is often used fortriglycerides analysis. With this detector, however, it takes a long time to stabilize the baseline and foreign substances often affect the results. ELSDis known as an effective detection method to solve the problems on fatty analysis including triglycerides, taking advantage of its high sensitivity and stable baseline.

PU- 2089
PU- 2089

Experimental

Results

Figure 1 shows the chromatogram of 6 well separated components of a triglycerides standard mixture.

Figure1. Chromatogram of 6 components of a triglycerides standard mixture. 1: Trilaurin, 2: Trilinorein, 3: Trimylistin, 4: Triolein, 5: Tripalmitin, 6: Tristearin

Figure 2 and Figure 3 show the chromatograms of rice bran oil and margarine, respectively.

Figure2. Chromatogram of rice bran oil. The peak numbers are the same as in Figure 1. Pre-treatment: 1. 0g of rice bran oil was dissolved in 10 mL of acetone and was then filtrated through a 0.45 µm membrane filter.
Figure3. Chromatogram of margarine. The peak numbers are the same as in Figure 1. Pre-treatment: 0.5 g of margarine was dissolved in 10mL of acetone and was then filtrated through a 0.45µm membrane filter.

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About the Author

DJ Tognarelli received his B.S. from the University of Richmond where he studied Analytical Chemistry. He is a Chromatography Applications Scientist at JASCO.