CD Measurement of α-pinene Gas in the Vacuum-UV Region

Download PDF May 26, 2017

Introduction

The J-1500 CD spectrometer has recently been updated to provide better signal detection and resolution for a variety of sample choices. The enhanced optical system increases the light throughput and the new electronics include the latest in digital lock-in technology, which allows for a small signal in a very noisy environment to be detected. These features ensure that CD spectra can obtained by the J-1500 for strongly absorbing and high signal-to-noise (S/N) samples across the spectrum and into the vacuum-UV region.

The CD spectra measurement in the vacuum-UV region of (1R)-(+)-α-pinene gas and (1S)-(-)-α-pinene gas is reported below.

J-1500 Circular Dichroism Spectrophotometer
J-1500 CD Spectrometer

Experimental

Measurement conditions 
 Data acquisition interval 0.1 nm
 Spectral bandwidth 1 nm
 Accumulations 1
 Scan speed 20 nm/min
 Response time 1 sec

Keywords

J-1500, circular dichroism, vacuum-UV region, α-pinene, gas measurement

Results

The vacuum-UV CD spectra of (1R)-(+)-α-pinene gas and (1S)-(-)-α-pinene gas are shown in Figure 1. The symmetrical CD spectra with high S/N were obtained at wavelengths as low as 163 nm and the sharp bands specific to gas samples can clearly be seen.

Figure 1. Vacuum-UV CD spectra of α-pinene gas

Conclusion

This application note demonstrates that gas samples can be obtained using the J-1500 CD spectrometer with high signalto-noise in the vacuum-UV region.

About the Author

Leah Pandiscia received her PhD from Drexel University where she studied Biophysical Chemistry. She is a Spectroscopy Applications Scientist at JASCO.