Skip to content

JASCO JASCO

  • News
  • Events
  • E-Store
  • My Account
  • Contact Us
  • Worldwide
Search
Click to view menu
MENUMENU
  • Products
    • Chromatography
      • HPLC
      • RHPLC
      • UHPLC
      • LC-MS
      • Preparative LC
      • Analytical SFC
      • Semi-Preparative SFC
      • Hybrid SFC
      • Fuel Analysis by SFC-FID
      • Preparative SFC
      • Supercritical Fluid Extraction
      • Chromatography Software
    • Molecular Spectroscopy
      • Circular Dichroism
      • High-Throughput CD
      • Vibrational CD
      • Circularly Polarized Luminescence (CPL)
      • Polarimeters
      • FTIR Spectrometers
      • FTIR Microscopy
      • FTIR Portable
      • Raman Microscopy
      • Palmtop Raman Spectrometer
      • Probe Raman
      • UV-Visible/NIR Spectrophotometers
      • UV-Visible/NIR Microscopy
      • Fluorescence Spectrophotometers
      • Film Thickness
      • Spectra Manager™ Suite
    • Refurbished
      • Refurbished HPLC Systems
      • HPLC Switching Valves
      • NRS-4100 Confocal Raman Microscopes
      • FTIR Accessories
  • Service
    • Service and Support Plans
    • Service Request Form
  • Applications
  • KnowledgeBase
  • Learning Center
    • Best Practices
      • Circular Dichroism Tips & Tricks for Biological Samples
      • CD Scale Calibration with ACS
      • Fluorescence Tips & Tricks
      • Raman Spectroscopy Tips & Tricks
    • Training Videos
      • ChromNAV
      • SF-NAV
      • Circular Dichroism
      • UV-Visible/NIR
      • Fluorescence
    • Training Seminars
      • Registration Form
    • Webinars
    • eBooks
    • Theory
      • Theory of Molecular Spectroscopy
      • Chromatography
  • About Us
    • President’s Message
    • Contact
    • History
    • Careers
  • News
  • Events
  • Worldwide
  • Shop
  • My Account

Home / Applications / Color Analysis using a UV-Visible Spectrophotometer

  • Industry

  • Technique

Color Analysis using a UV-Visible Spectrophotometer

By Leah Pandiscia, PhD

image_pdfView and Download PDF
August 15, 2022

Introduction

UV-Visible/NIR Spectrophotometer
V-750 UV-Visible/NIR Spectrophotometer

Color is a visually perceived property that is derived from the reflected or transmitted spectrum of light interacting with the human eye. It is important for the development and production of color-based products to evaluate and obtain measurable factors that describe the color. JASCO has developed a variety of  color analysis using a UV-Visible spectrophotometer for both reflectance and/or transmittance spectra of samples. The V-700 spectrophotometers have a double-beam optical system that provides high measurement accuracy and stability, which  results in color evaluation that can be determined with high reproducibility.

Mechanism of color recognition by the human eye
Figure 1. Mechanism of color recognition by the human eye.

This application note tests the reproducibility of color evaluation made using JASCO’s color diagnosis system.

Experimental

Measurements were obtained for blue, red, and yellow pellets. Baseline measurement was performed using a standard white reference plate and then the sample was measured. Both reference and sample measurements were made with the specular component included. To confirm the measurement reproducibility 10 spectra were collected with the sample removed and replaced in between scans to assess the effects of changing the measurement position.

Keywords

UV-0044, V-700, UV-Visible/NIR, Chromaticity, Color Analysis, Material, ISV-922 Integrating Sphere, Color Diagnosis program

Results

The reflectance spectra for each sample are shown in Figure 2. The figure on the right shows zoomed-in spectra in the wavelength range 740 – 780 nm. As shown in each spectrum, the difference between maxima and minima of the photometric value is less than 0.15%R, indicating high reproducibility measurements.

Reflectance spectra of the three colored pellets. The figures on the right are the zoomed in spectra from 740 to 780 nm
Figure 2. Reflectance spectra of the three colored pellets. The figures on the right are the zoomed in spectra from 740 to 780 nm.

The Color Diagnosis Program was used to calculate color coordinates from the measured reflectance spectra. Figure 3 shows the results plotted on XYZ, L*a*b*, Lab, and L*u*v* chromaticity diagrams. The calculated values are shown in Tables 1, 2 and 3 for the blue, red, and yellow pellets, respectively. For the color coordinates that represent chroma and hue (xy, a*b*, ab, u*v*), the difference between maxima and the minima are; x and y, less than 0.0012, a* and b*, less than 0.31, a and b, less than 0.18, u* and v*, less than 0.50. For the color coordinates that represent brightness (Y, L, L*), the difference between the maxima and minima is less than 0.1. All results indicate highly reproducible chromaticity values using the Color Diagnosis system.

Chromaticity diagrams for each color system and pellet
Figure 3. Chromaticity diagrams for each color system and pellet.

Table 1. Color calculation results of blue color pellet.

XYZxyL*a*b*Labu*v*
123.7723.9465.840.20940.210856.034.58-44.9448.933.78-50.19-24.75-70.73
223.7723.9465.810.20940.210956.034.57-44.9148.933.78-50.15-24.74-70.69
323.7423.9165.700.20950.210955.994.57-44.8848.893.78-50.09-24.71-70.62
423.7623.9365.720.20950.211056.014.57-44.8748.913.78-50.08-24.71-70.60
523.7623.9265.710.20950.211056.014.56-44.8548.913.77-50.06-24.71-70.58
623.7923.9665.850.20940.210956.044.57-44.9248.953.78-50.16-24.75-70.70
723.8023.9765.850.20950.211056.064.55-44.8948.963.77-50.12-24.74-70.65
823.8123.9865.830.20950.211156.074.55-44.8748.973.76-50.08-24.73-70.62
923.8023.9765.790.20960.211156.064.55-44.8548.963.76-50.06-24.71-70.58
1023.8023.9765.810.20950.211156.064.55-44.8648.963.76-50.08-24.73-70.61
Max-Min0.070.070.150.00020.00030.070.030.090.070.020.130.050.14
Ave23.7823.9565.790.210.2156.044.56-44.8848.943.77-50.11-24.73-70.64
SD0.022890.025090.058790.000060.000080.02520.01040.03120.02560.00830.04560.01680.0512
CV%0.100.100.090.030.040.40.23-0.070.050.22-0.09-0.07-0.07

Table 2. Color calculation results of red color pellet.

XYZxyL*a*b*Labu*v*
127.7417.168.170.52260.323448.4753.7826.7841.4349.9915.67101.0519.17
227.7817.178.090.52380.323748.4753.9427.0841.4450.1715.80101.5519.40
327.7617.158.100.52360.323648.4553.9227.0241.4150.1415.77101.4619.34
427.7517.158.080.52380.323748.4553.9227.0641.4150.1315.79101.4819.37
527.7317.148.100.52360.323648.4353.9026.9941.4050.1115.75101.3919.31
627.7017.118.100.52350.323548.4053.8926.9441.3750.1015.73101.3519.27
727.7617.188.130.52310.323748.4953.8026.9741.4550.0115.76101.2119.34
827.7217.148.110.52330.323548.4353.8526.9341.4050.0615.73101.2719.27
927.7217.148.110.52330.323548.4353.8626.9341.4050.0615.73101.2819.27
1027.7717.198.130.52310.323848.5053.7826.9741.4650.0015.76101.1819.35
Max-Min0.090.080.080.00120.00040.100.160.310.090.180.130.500.23
Ave27.7417.158.110.520.3248.4553.8626.9741.4250.0815.75101.3219.31
SD0.02700.02300.02740.000380.000120.02880.06130.08520.02780.06400.03770.15450.0670
CV %0.100.130.340.070.040.060.110.320.070.130.240.150.35

Table 3. Color calculation results of yellow color pellet.

XYZxyL*a*b*Labu*v*
127.7417.168.170.52360.323448.4753.7826.7841.4349.9915.67101.0519.17
227.7817.178.090.52380.323748.4753.9427.0841.4450.1715.80101.5519.40
327.7617.158.100.52360.323648.4553.9227.0241.4150.1415.77101.4619.34
427.7517.158.080.52380.323748.4553.9227.0641.4150.1315.79101.4819.37
527.7317.148.100.52360.323648.4353.9026.9941.4050.1115.75101.3919.31
627.7017.118.100.52350.323548.4053.8926.9441.3750.1015.73101.3519.27
727.7617.188.130.52310.323748.4953.8026.9741.4550.0115.76101.2119.34
827.7217.148.110.52330.323548.4353.8526.9341.4050.0615.73101.2719.27
927.7217.148.110.52330.323548.4353.8626.9341.4050.0615.73101.2819.27
1027.7717.198.130.52310.323848.5053.7826.9741.4650.0015.76101.1819.35
Max-Min0.090.080.080.00120.00040.100.160.310.090.180.130.500.23
Ave.27.7417.158.110.520.3248.4553.8626.9741.4250.0815.75101.3219.31
SD0.02700.02300.02740.000380.000120.02880.06130.08520.02780.06400.03770.15450.0670
CV%0.100.130.340.070.040.060.110.320.070.130.240.150.35

Analysis Conditions: Light Source, D65 (CIE 15:2004, JIS Z 8781-1:2012, ISO 11664-2:2007); Color-matching function, CIE 15:2004, JIS Z 8701:1982; Viewing angle, 2º; Data interval, 5 nm.

image_pdfView and Download PDF

Featured Products:

  • V-730 UV-Visible/NIR Spectrophotometer

    A general-purpose double-beam spectrophotometer with a compact space saving design

    V-730 UV-Visible Spectrophotometer

  • V-750 UV-Visible/NIR Spectrophotometer

    A high resolution UV-Visible double-beam spectrophotometer with single monochromator, variable SBW and PMT detector

    V-750 UV-Visible Spectrophotometer

About the Author

Leah Pandiscia received her PhD from Drexel University where she studied Biophysical Chemistry. She is a Spectroscopy Applications Scientist.

JASCO provides services and support to a diverse range of industries throughout the world.

View our support plans

Connect with JASCO

  • Facebook
  • Twitter
  • LinkedIn
  • JASCO Sales
  • 800-333-5272

Receive the latest promotions and special offers

  • Careers
  • Press Kit
  • Privacy Policy
  • Sitemap

© , JASCO. All Rights Reserved.