Melamine is an organic nitrogen compound which has a triazine ring in the center of its structure. It is used as the material of melamine resin which has high thermal and water resistance and is used for mechanical strengthening. Recently, there was a case that melamine was used as an additive to diluted milk to make-up for the lack protein content, however, this milk product caused health problems. The Food and Drug Administration (FDA) has been evaluating the risk of melamine and has assigned HPLC with UV detection as one of the analysis methods of melamine in food.
In this paper, the analysis results of melamine in a milk product was measured using a PDA detector.
Figure 1 shows the structure of melamine, while figure 2 shows the chromatogram and contour plot of melamine.
Figure 3 shows the on peak spectrum of the melamine standard sample.
Figure 4 shows the chromatogram and on peak spectrum of milk spiked with melamine. By registering the spectrum of the standard sample in figure 3, the correlation coefficient was calculated to be 1.000.