High Speed Analysis of Melamine in Dairy Products by HPLC

Download PDF August 19, 2022


Melamine is an organic nitrogen compound which has a triazine ring in the center of its structure. It is used as the material of melamine resin which has high thermal and water resistance and is used for mechanical strengthening. Recently, there was a case that melamine was used as an additive to diluted milk to make-up for the lack protein content, however, this milk product caused health problems. The Food and Drug Administration (FDA) has been evaluating the risk of melamine and has assigned HPLC with UV detection as one of the analysis methods of melamine in food.

In this paper, the analysis results of melamine in a milk product was measured using a PDA detector.



Figure 1 shows the structure of melamine, while figure 2 shows the chromatogram and contour plot of melamine.

Figure 1 Structure of melamine
Figure 2 Chromatogram of melamine
1. Melamine

Figure 3 shows the on peak spectrum of the melamine standard sample.

Figure 3 On peak spectrum of the melamine standard sample.

Figure 4 shows the chromatogram and on peak spectrum of milk spiked with melamine. By registering the spectrum of the standard sample in figure 3, the correlation coefficient was calculated to be 1.000.

Figure 4 Chromatogram of milk spiked with melamine.
Sample preparation: 20 µL of 100 µg/L melamine solution was added to 180 µL of milk, diluted with 1800 µL of ultrapure water and added to 200 µL of eluent. The solution was centrifuged at 5000 rpm for 10 minutes and then the supernatant was filtrated using 0.45 µm membrane filter.

About the Author

Keisuke Watanabe