High Speed Separation of Components in Cold Medicine using UHPLC

By Miyuki Kanno

PDF IconDownload This Application

January 5, 2024

Introduction

The main components of a common cold medicine includes acetoaminophen, anhydrous caffeine, hesperidine, ethenzamide, tipepidinehibenzate, andapronalid. We examined the applicability of an X-Press Pak C18S column (2.1mm .I.D. x 50mmL.) packed with 2µm diameter packing material for the ultra-high speed separation of the above medicines. The results were examined to determine whether the performance of the column and chromatography separation exceeds that of conventional HPLC.

JASCO X-LC system

Experimental

The X-LC system utilized in this experiment wa sa JASCOX-LC system consisting of a 3185PU pump, 3080DG degasser, 3067CO column oven, 3070UV UV/Vis detector, 3059AS auto sampler and a chromatography data system.

Keywords

745023X

Results

Figure 1 shows the separation of a standard mixture including acetoaminophen (0.02mg / mL), anhydrouscaffeine (0.02mg / mL), phenol (for internal standard, 0.02mg/mL), hesperidine (0.02mg/mL), ethenzamide (0.02mg/mL), tipepidinehibenzate(0.02 mg/mL) andapronalid (0.1mg/mL).TheX-LC system provides an analysis time 6 times faster than conventional HPLC without sacrificing the resolution between each peak.

Figure1 Chromatogram of a standardmixture of components of cold medicines Peaks: 1 = acetoaminophen (0.02mg/mL), 2 = anhydrouscaffeine (0.02mg/mL), 3 = phenol(internalstandard,0.02mg/mL), 4 = hesperidin(0.02mg/mL), 5 = ethenzamide(0.02 mg/mL), 6 = tipepidinehibenzate(0.02mg/mL), and 7 = apronalid(0.1mg/mL) Chromatografic conditions: column = X-PressPak C18S (2.1mmI.D.x50mmL.) ,column temperature = 40ºC, mobile phase = methanol/0.1% phosphoric acid (40/60), flow rate = 0.5 mL/min, detection wave length = 260nm, injection volume = 1µL
This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

Featured Products:

About the Author

Miyuki Kanno