High Speed Separation of Disulfiram using UHPLC
January 5, 2024
Introduction
Disulfiram is a drug used to treat chronic alcoholism by producing an acute sensitivity to alcohol. We examined the utility of an X-Press PakC18S column (2.1mm I.D. x 50mmL.) packed with 2µm diameter packing material for the ultra-high speed separation of the above drug. The results were examined to determine whether the performance of the column and chromatography separation exceed those of conventional HPLC.
![](https://jascoinc.com/wp-content/uploads/2014/01/JASCO-X-LC-system.png)
Experimental
The chromatography system utilized in this experiment was a JASCOX-LC system consisting of a 3185P UHPLC pump, 3080DG degasser, 3067CO column oven, 3070UV UV/Vis detector, Model 3059AS auto sampler and a chromatography data system.
Keywords
747007X
Results
Figure 1 shows the separation of a standard mixture of benzophenone (0.005mg/mL) and disulfiram (0.005mg/mL). The X-LC system provides an analysis time 7 times shorter than conventional HPLC while the resolution between the benzophenone and disulfiram was 5.4; the reproducibility of the peak ratio is 0.44%. These results well exceed those of conventional HPLC.
![](https://jascoinc.com/wp-content/uploads/2013/12/Figure-1-3.png)
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High Speed Separation of Disulfiram using UHPLC
Introduction
Disulfiram is a drug used to treat chronic alcoholism by producing an acute sensitivity to alcohol. We examined the utility of an X-Press PakC18S column (2.1mm I.D. x 50mmL.) packed with 2µm diameter packing material for the ultra-high speed separation of the above drug. The results were examined to determine whether the performance of the column and chromatography separation exceed those of conventional HPLC.
![](https://jascoinc.com/wp-content/uploads/2014/01/JASCO-X-LC-system.png)
Experimental
The chromatography system utilized in this experiment was a JASCOX-LC system consisting of a 3185P UHPLC pump, 3080DG degasser, 3067CO column oven, 3070UV UV/Vis detector, Model 3059AS auto sampler and a chromatography data system.
Keywords
747007X
Results
Figure 1 shows the separation of a standard mixture of benzophenone (0.005mg/mL) and disulfiram (0.005mg/mL). The X-LC system provides an analysis time 7 times shorter than conventional HPLC while the resolution between the benzophenone and disulfiram was 5.4; the reproducibility of the peak ratio is 0.44%. These results well exceed those of conventional HPLC.
![](https://jascoinc.com/wp-content/uploads/2013/12/Figure-1-3.png)