Polycyclic aromatic hydrocarbons (PAHs), which consist of fused aromatic rings, are produced by incomplete combustion of carbon-containing fuels such as diesel and coal. Some of them are suspected to be carcinogens, requiring the analysis of environmental PAHs. In this paper, we examine the utility of the JASCO X-LC system for the separation and determination of PAHs.
The UHPLC system utilized in this experiment was a JASCO X-LC system consisting of two PU-3185 pumps, a DG-3080 mobile phase degasser, a MX-3080 mixing unit, a CO-3067 column oven, an FP-3120 fluorescence detector, an AS-3059 autosampler, and the ChromNAV chromatography data system. The column used is a Zorbax Eclipse PAH (2.1 mm ID x 50 mmL, 1.8 µm). PAHs from the residue in a diesel engine was extracted for 8 hours using a soxhlet apparatus with dichloromethane as the extraction solvent.
Figure 1 is an X-LC chromatogram of a standard mixture of 15 components of PAHs (200 pg each). The chromatograms were obtained using gradient elution and a time program of excitation and emission wavelengths for the fluorescence detector. The X-LC system provides an analysis time 3X shorter than a conventional HPLC separation. Figure 2 outlines an X-LC chromatogram obtained from the diesel engine residue with eight components separated with satisfactory resolution.