Recycling Preparative HPLC of Polystyrene Oligomer

Download PDF August 25, 2017

Introduction

Recycling Preparative HPLC is a separation technique that can achieve complete separation of a number of components by repeatedly cycling the eluent to the same column. This has the same effect as using longer or multiple columns. When applied to preparative HPLC each purified component can be fractionated very effectively.

This technique has the following features:
(1) Reduced column costs, as it is not necessary to use longer or multiple columns.
(2) Reduces environmental loads and costs, because in recycle mode, the same mobile phase solvent is used repeatedly while improving separation.
(3) Effective preparative purification because each component is fractionated only after improving the separation.

Jasco PU-2086
Jasco PU-2086

Experimental

Equipment

Eluent Pump: PU-2086
Autosampler: AS-2058
Detector:UV-2070 (with semi-prep. cell)
Fraction Collector:ADVANTEC CHF122SC
Fraction Controller FC-2088-30
Fraction Valve unit HV-2088-06

Conditions

ColumnMegapak GEL 201C (20mm ID x 500 mmL)
Eluent:Chloroform
Eluent Flow Rate:3.0 mL/min
Wavelength:254 nm
Injection Volume:200 uL
Standard Sample:Polystyrene Oligomer (1.0 %(w/v) in Chloroform)
Recycle Kit
Fig. 1. Flow Diagram of Recycling Preparative HPLC system

Results

Fig. 2 Recycle chromatogram of a Polystyrene Oligomer, showing the improvement of separation by recycling six times in approximately 180 minutes. Three components tetramer, pentamer and hexamer of Polystyrene Oligomer were clearly separated and fractionated.

Recycle Chromatogram of Polystyrene Oligomer
Fig. 2. Recycle Chromatogram of Polystyrene Oligomer

About the Author

Masao Bounashita