Skip to content

JASCO JASCO

  • News
  • Events
  • E-Store
  • My Account
  • Contact Us
  • Worldwide
Search
Click to view menu
MENUMENU
  • Products
    • Chromatography
      • HPLC
      • RHPLC
      • UHPLC
      • LC-MS
      • Preparative LC
      • Analytical SFC
      • Semi-Preparative SFC
      • Hybrid SFC
      • Fuel Analysis by SFC-FID
      • Preparative SFC
      • Supercritical Fluid Extraction
      • Chromatography Software
    • Molecular Spectroscopy
      • Circular Dichroism
      • High-Throughput CD
      • Vibrational CD
      • Circularly Polarized Luminescence (CPL)
      • Polarimeters
      • FTIR Spectrometers
      • FTIR Microscopy
      • FTIR Portable
      • Raman Microscopy
      • Palmtop Raman Spectrometer
      • Probe Raman
      • UV-Visible/NIR Spectrophotometers
      • UV-Visible/NIR Microscopy
      • Fluorescence Spectrophotometers
      • Film Thickness
      • Spectra Manager™ Suite
    • Refurbished
      • Refurbished HPLC Systems
      • HPLC Switching Valves
      • NRS-4100 Confocal Raman Microscopes
      • FTIR Accessories
  • Service
    • Service and Support Plans
    • Service Request Form
  • Applications
  • KnowledgeBase
  • Learning Center
    • Best Practices
      • Circular Dichroism Tips & Tricks for Biological Samples
      • CD Scale Calibration with ACS
      • Fluorescence Tips & Tricks
      • Raman Spectroscopy Tips & Tricks
    • Training Videos
      • ChromNAV
      • SF-NAV
      • Circular Dichroism
      • UV-Visible/NIR
      • Fluorescence
    • Training Seminars
      • Registration Form
    • Webinars
    • eBooks
    • Theory
      • Theory of Molecular Spectroscopy
      • Chromatography
  • About Us
    • President’s Message
    • Contact
    • History
    • Careers
  • News
  • Events
  • Worldwide
  • Shop
  • My Account

Home / Applications / Analysis of Sugars by HPLC-ELSD

  • Industry

  • Technique

Analysis of Sugars by HPLC-ELSD

By Toshifumi Uchiyama

image_pdfView and Download PDF
August 19, 2022

Introduction

Sugar Analysis HPLC
PU- 2089

An evaporative light scattering detector (ELSD) is a universal HPLC detector whose detection principle is based on the phenomenon of light scattering, which occurs from particles of residual non-volatile components after removing the volatile mobile phase using a combination of heat and gas nebulization.  The ELSD detector uses an LED as the light source and a photomultiplier to measure change in intensity as the analyte passes through the optical path. Compounds that are typically measured using a refractive index detector or a short wavelength of a UV detector may be measured with increased sensitivity or a more stable baseline using ELSD.

In this application note, monosaccharides and disaccharides were analyzed using ELSD from a separation using a polymer NH2 column in HILIC mode.

Experimental

Instrument

PumpPU-2089S
AutosamplerAS-2057
Column OvenCO-2060
DetectorELSD-2040

Method

ColumnAsahiPak NH2P-50 4E
(4.6 ID x 250 L mm)
EluentH2O/ACN 25:75
Flow Rate1.0 mL/min
Column Temperature30 deg C
ELSD ParametersNebulizer temp. 30 deg C
Evaporator temp. 30 deg C
Gas flow rate 1.4 SLM
Injection Volume10uL
Standard SamplesRhamnose, Fructose, GLucose, Sucrose, Maltose
1.0mg/mL in H2O/ACN 50:50

Keywords

Sugar, HILIC, Polymer NH2 column, ELSD, Rhamnose, Fructose, Glucose, Sucrose, Maltose, monosaccharides

Results

Figure 1. Chromatogram of a standard mixture of monosaccharides and disaccharides.

Figure 1. Chromatogram of a standard mixture of sugars.
1: Rhamnose, 2: Fructose, 3: Glucose, 4: Sucrose, 5: Maltose
image_pdfView and Download PDF

Featured Products:

  • Single Quad LC-MS

  • Compact HPLC

  • MD-4010 PDA

    HPLC Detectors

About the Author

Toshifumi Uchiyama is a member of the electronic spectroscopy team located at the JASCO main laboratory in Tokyo

JASCO provides services and support to a diverse range of industries throughout the world.

View our support plans

Connect with JASCO

  • Facebook
  • Twitter
  • LinkedIn
  • JASCO Sales
  • 800-333-5272

Receive the latest promotions and special offers

  • Careers
  • Press Kit
  • Privacy Policy
  • Sitemap

© , JASCO. All Rights Reserved.