An evaporative light scattering detector (ELSD) is a universal HPLC detector whose detection principle is based on the phenomenon of light scattering, which occurs from particles of residual non-volatile components after removing the volatile mobile phase using a combination of heat and gas nebulization. The ELSD detector uses an LED as the light source and a photomultiplier to measure change in intensity as the analyte passes through the optical path. Compounds that are typically measured using a refractive index detector or a short wavelength of a UV detector may be measured with increased sensitivity or a more stable baseline using ELSD.
In this application note, monosaccharides and disaccharides were analyzed using ELSD from a separation using a polymer NH2 column in HILIC mode.
|Column||AsahiPak NH2P-50 4E|
(4.6 ID x 250 L mm)
|Flow Rate||1.0 mL/min|
|Column Temperature||30 deg C|
|ELSD Parameters||Nebulizer temp. 30 deg C|
Evaporator temp. 30 deg C
Gas flow rate 1.4 SLM
|Standard Samples||Rhamnose, Fructose, GLucose, Sucrose, Maltose|
1.0mg/mL in H2O/ACN 50:50
Sugar, HILIC, Polymer NH2 column, ELSD, Rhamnose, Fructose, Glucose, Sucrose, Maltose, monosaccharides
Figure 1. Chromatogram of a standard mixture of monosaccharides and disaccharides.