Introduction

An evaporative light scattering detector (ELSD) is a universal HPLC detector whose detection principle is based on the phenomenon of light scattering, which occurs from particles of residual non-volatile components after removing the volatile mobile phase using a combination of heat and gas nebulization.  The ELSD detector uses an LED as the light source and a photomultiplier to measure change in intensity as the analyte passes through the optical path. Compounds that are typically measured using a refractive index detector or a short wavelength of a UV detector may be measured with increased sensitivity or a more stable baseline using ELSD.

In this application note, monosaccharides and disaccharides were analyzed using ELSD from a separation using a polymer NH2 column in HILIC mode.

Experimental

Instrument

Method

Keywords

Sugar, HILIC, Polymer NH2 column, ELSD, Rhamnose, Fructose, Glucose, Sucrose, Maltose, monosaccharides

Results

Figure 1. Chromatogram of a standard mixture of monosaccharides and disaccharides.