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Home / Applications / Ultra High-Speed Analysis of Alkylphenones by UHPLC

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  • Technique

Ultra High-Speed Analysis of Alkylphenones by UHPLC

By Satoko Suzuki

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January 5, 2024

Introduction

Alkylphenones are often used for evaluating HPLC systems because of their reasonable retention times on ODS columns and easy separation with a water/acetonitrile mobile phase.

This report outlines the ultra-high speed analysis of alkylphenones analysis by Ultra High-performance Liquid Chromatography (UHPLC) using with a PDA detector (high-speed data acquisition of 100 spectra/sec).

X-LC system

Experimental

Keywords

032001X

Results

Figure 1 3D chromatogram of an alkylphenone standard mixture containing 9 components. All of the alkylphenones were successfully separated and detected within 12 seconds.

Figure 1. 3D Chromatogram of standard alkylphenones.

1: Acetanilide, 2: Acetophenone,3: Propiophenone, 4: Butyrophenone, 5: Benzophenone, 6: Valerophenone, 7: Hexanophenone, 8: Heptanophenone, 9: Octanophenone.

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

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About the Author

Satoko Suzuki is a senior manager of the molecular spectroscopy applications laboratory at the JASCO facility in Hachioji Japan.

JASCO Application Note

Ultra High-Speed Analysis of Alkylphenones by UHPLC

Introduction

Alkylphenones are often used for evaluating HPLC systems because of their reasonable retention times on ODS columns and easy separation with a water/acetonitrile mobile phase.

This report outlines the ultra-high speed analysis of alkylphenones analysis by Ultra High-performance Liquid Chromatography (UHPLC) using with a PDA detector (high-speed data acquisition of 100 spectra/sec).

X-LC system

Experimental

Results

Figure 1 3D chromatogram of an alkylphenone standard mixture containing 9 components. All of the alkylphenones were successfully separated and detected within 12 seconds.

Figure 1. 3D Chromatogram of standard alkylphenones.

1: Acetanilide, 2: Acetophenone,3: Propiophenone, 4: Butyrophenone, 5: Benzophenone, 6: Valerophenone, 7: Hexanophenone, 8: Heptanophenone, 9: Octanophenone.

Keywords

032001X

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.
28600 Mary’s Court, Easton, MD 21601 USA • (800) 333-5272 • Fax: (410) 822-7526 • jascoinc.com/applications

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