Ultra High-Speed Analysis of Curcumin in Turmeric by UHPLC

Download PDF August 25, 2017

Introduction

Curcumin is the principal curucuminoid of the popular Indian spice turmeric, which is a member of the polyphenols. It is well known that it has physiological effects such as anti-ulcer, antioxidant and anti-inflammatory activities. Turmeric curucuminoids contain curcumin as keto and enol types.

In this report, curcuminoids in turmeric were analyzed using Ultra High-Performance Liquid Chromatography (UHPLC) with PDA detector, which enables ultra high-speed data aquisition of 100 spectra/sec.

JASCO X-LC system

Experimental

Results

Figure 1 shows the chromatogram and contour plot of the curcuminoid standard mixture. The components were clearly separated within 1 minute.

Figure 1. Chromatogram of the Curcumin standard mixture. 1: Bis-demethoxycurcumin, 2: Demethoxycurcumin, 3: Curcumin

Figure 2 shows the on-peak spectra of the components of the curcuminoid standard. High quality spectra were obtained for all three components.

Figure 2. On-peak spectra of the Curcumin standard. 1: Bis-demethoxycurcumin, 2: Demethoxycurcumin, 3: Curcumin

Figure 3 shows the chromatogram of commercial turmeric and the on-peak spectrum of each peak. By registering the spectra of standard components in figure 2, the correlation coefficient was calculated to be as good as 1.000.

Figure 3. Chromatogram of commercial turmeric. 1: Bis-demethoxycurcumin, 2: Demethoxycurcumin, 3: Curcumin

Preparation: 500 milligrams of turmeric was first dissolved in 5 ml of acetonitrile. After sonication, the supernatant was filtered using 0.45 µm membrane filter, the filtrate was diluted with pure water (1:1) and it was filtered again using 0.2 µm membrane filter.

About the Author

Masao Bounashita