Skip to content

JASCO JASCO

  • News
  • Events
  • E-Store
  • My Account
  • Contact Us
  • Worldwide
Search
Click to view menu
MENUMENU
  • Products
    • Chromatography
      • HPLC
      • RHPLC
      • UHPLC
      • LC-MS
      • Preparative LC
      • Analytical SFC
      • Semi-Preparative SFC
      • Hybrid SFC
      • Fuel Analysis by SFC-FID
      • Preparative SFC
      • Supercritical Fluid Extraction
      • Chromatography Software
    • Molecular Spectroscopy
      • Circular Dichroism
      • High-Throughput CD
      • Vibrational CD
      • Circularly Polarized Luminescence (CPL)
      • Polarimeters
      • FTIR Spectrometers
      • FTIR Microscopy
      • FTIR Portable
      • Raman Microscopy
      • Palmtop Raman Spectrometer
      • Probe Raman
      • UV-Visible/NIR Spectrophotometers
      • UV-Visible/NIR Microscopy
      • Fluorescence Spectrophotometers
      • Film Thickness
      • Spectra Manager™ Suite
    • Refurbished
      • Refurbished HPLC Systems
      • HPLC Switching Valves
      • FTIR Accessories
  • Service
    • Service and Support Plans
    • Service Request Form
  • Applications
  • KnowledgeBase
  • Learning Center
    • Best Practice
      • Circular Dichroism Tips & Tricks for Biological Samples
      • CD Scale Calibration with ACS
      • Fluorescence Tips & Tricks
      • Raman Spectroscopy Tips & Tricks
    • Training Videos
      • ChromNAV
      • SF-NAV
      • Circular Dichroism
      • UV-Visible/NIR
      • Fluorescence
    • Training Seminars
      • Training Registration Form
    • Webinars
    • eBooks
    • Theory
      • Theory of Molecular Spectroscopy
      • Chromatography
  • About Us
    • President’s Message
    • Contact
    • History
    • Careers
  • News
  • Events
  • Worldwide
  • Shop
  • My Account
  • Contact Us

Home / Applications / Transmission Measurement of Volvox Algae using a MSV-5000 Series UV-Visible Microscope

  • Industry

  • Technique

Transmission Measurement of Volvox Algae using a MSV-5000 Series UV-Visible Microscope

By Heather Haffner

PDF IconDownload This Application

January 5, 2024

Introduction

MSV-5200 UV-Visible/NIR Microscopic Spectrophotometer
MSV-5200
UV-Visible/NIR Microscopic
Spectrophotometer

The MSV-5000 Series microscopic spectrophotometer is for transmission and reflection measurements in a wide wavelength range from the ultraviolet to near infrared. The built-in high resolution camera enables sample areas as small as 10 mm in diameter to be precisely measured and is therefore gaining popularity in the bioscience field for analyzing localized constituents in living cells.

Volvox is a form of algae which forms localized, spherical colonies of up to 50,000 cells in the mother colony. In this application note, a daughter colony of Volvox was measured to obtain the absorption spectra and fixed-wavelength mapping.

Fig. 1. Dried volvox.

Volvox, has a localized cellular density due to its internal daughter colonies and was measured to obtain absorption spectra and fixed wavelength mapping data.

Experimental

Measurement Conditions
AbsorptionFixed Wavelength Mapping
UV-Vis Bandwidth5 nmMode/WavelengthLattice/672 nm
Scan Speed1000 nm/minBandwidth2 nm
ResponseQuickResponseFast
Data Interval1 nmData Interval30 μm
Cassegrain Objective16xCassegrain Objective16x
Aperture50 mmΦAperture30 μmΦ

Keywords

210-UV-0024, MSV-5100 UV-Vis microscopic spectrophotometer, MAXY-501 Automatic XYZ stage, Biochemistry

Results

Figure 2. Absorption spectrum of Volvox (right) and chlorophyll a and b (right)1.

The measured absorption spectrum of Volvox is shown in Figure 2 on the left. On the right the absorption spectra of chlorophyll a and b are shown, which are major components of algae1 and therefore Volvox. The published literature data is measured under acetone solvent conditions and depending on the solvent conditions, the peak positions of chlorophylls can be shifted 2-7 nm. However, when comparing the obtained Volvox spectrum to the literature chlorophyll spectra it can be assumed that Vovlox contains both chlorophyll a and b.

Figure 3. Observation image (left) and color-coded diagram of mapping measurement (right) of mapping.

Fixed wavelength mapping measurements were obtained at 672 nm, the peak observed in the absorption spectrum in Figure 2. The high speed mapping images are shown below in Figure 3. The areas with higher cellular density are easily visualized in both the sample observation image and color-coded diagram.

References

1. Tanaka, Ryouichi and Ayumi Tanaka. “Chlorophyll cycle regulates the construction and destruction of the light-harvesting complexes.” Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1807, 9, 2011, 968-976.

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

Featured Products:

  • UV-visible (NIR) Microscopes

  • Modular UV-visible/NIR Spectrometer System

    MV-3000 Series Portable UV-Visible/NIR Spectrophotometer

  • A high sensitivity UV-Visible/NIR Spectrophotometer with InGaAs detector for wavelengths up to1600nm

    V-780 UV-Visible/NIR Spectrophotometer

About the Author

JASCO Application Note

Transmission Measurement of Volvox Algae using a MSV-5000 Series UV-Visible Microscope

Introduction

MSV-5200 UV-Visible/NIR Microscopic Spectrophotometer
MSV-5200
UV-Visible/NIR Microscopic
Spectrophotometer

The MSV-5000 Series microscopic spectrophotometer is for transmission and reflection measurements in a wide wavelength range from the ultraviolet to near infrared. The built-in high resolution camera enables sample areas as small as 10 mm in diameter to be precisely measured and is therefore gaining popularity in the bioscience field for analyzing localized constituents in living cells.

Volvox is a form of algae which forms localized, spherical colonies of up to 50,000 cells in the mother colony. In this application note, a daughter colony of Volvox was measured to obtain the absorption spectra and fixed-wavelength mapping.

Fig. 1. Dried volvox.

Volvox, has a localized cellular density due to its internal daughter colonies and was measured to obtain absorption spectra and fixed wavelength mapping data.

Experimental

Measurement Conditions
AbsorptionFixed Wavelength Mapping
UV-Vis Bandwidth5 nmMode/WavelengthLattice/672 nm
Scan Speed1000 nm/minBandwidth2 nm
ResponseQuickResponseFast
Data Interval1 nmData Interval30 μm
Cassegrain Objective16xCassegrain Objective16x
Aperture50 mmΦAperture30 μmΦ

Results

Figure 2. Absorption spectrum of Volvox (right) and chlorophyll a and b (right)1.

The measured absorption spectrum of Volvox is shown in Figure 2 on the left. On the right the absorption spectra of chlorophyll a and b are shown, which are major components of algae1 and therefore Volvox. The published literature data is measured under acetone solvent conditions and depending on the solvent conditions, the peak positions of chlorophylls can be shifted 2-7 nm. However, when comparing the obtained Volvox spectrum to the literature chlorophyll spectra it can be assumed that Vovlox contains both chlorophyll a and b.

Figure 3. Observation image (left) and color-coded diagram of mapping measurement (right) of mapping.

Fixed wavelength mapping measurements were obtained at 672 nm, the peak observed in the absorption spectrum in Figure 2. The high speed mapping images are shown below in Figure 3. The areas with higher cellular density are easily visualized in both the sample observation image and color-coded diagram.

Keywords

210-UV-0024, MSV-5100 UV-Vis microscopic spectrophotometer, MAXY-501 Automatic XYZ stage, Biochemistry

References

1. Tanaka, Ryouichi and Ayumi Tanaka. “Chlorophyll cycle regulates the construction and destruction of the light-harvesting complexes.” Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1807, 9, 2011, 968-976.

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.
28600 Mary’s Court, Easton, MD 21601 USA • (800) 333-5272 • Fax: (410) 822-7526 • jascoinc.com/applications

Close

Designed in Tokyo. TRUSTED globally.

View our support plans

Connect with JASCO

  • Facebook
  • Twitter
  • LinkedIn
  • JASCO Sales
  • 800-333-5272

Receive the latest promotions and special offers

  • This field is for validation purposes and should be left unchanged.
  • Careers
  • Press Kit
  • JASCO Privacy Policy
  • Sitemap
  • Environmental Policy

© , JASCO. All Rights Reserved.