Title
A cationic fluorescent polymeric thermometer for the ratiometric sensing of intracellular temperature
Author
Seiichi Uchiyama, Toshikazu Tsuji, Kumiko Ikado, Aruto Yoshida, Kyoko Kawamoto, Teruyuki Hayashi, Noriko Inada
Year
2015
Journal
Analyst
Abstract
We developed new cationic fluorescent polymeric thermometers containing both benzothiadiazole and BODIPY units as an environment-sensitive fluorophore and as a reference fluorophore, respectively. The temperature-dependent fluorescence spectra of the thermometers enabled us to perform highly sensitive and practical ratiometric temperature sensing inside living mammalian cells. Intracellular temperatures of non-adherent MOLT-4 (human acute lymphoblastic leukaemia) and adherent HEK293T (human embryonic kidney) cells could be monitored with high temperature resolutions (0.01–1.0 °C) using the new cationic fluorescent polymeric thermometer.
Instrument
FP-6500
Keywords
Fluorescence, Thermal stability, Chemical stability, Quantum yield, Materials