Title
A collagen telopeptide binding peptide shows potential in aiding collagen bundle formation and fibril orientation
Author
Wenyu Yang, Lin Li, Guanghao Su, Zhe Zhang, Yiting Cao, Xuemin Li, Yanping Shi, Qiqing Zhang
Year
2017
Journal
Biomaterials Science
Abstract
Type I collagen, the main component of the extracellular matrix (ECM), assembles into bundles, which are then arranged in an orderly manner in many tissues. Various in vitromethods have been developed to mimic this native characteristic to create aligned, collagen-based materials for constructing tissue-engineered organs. In this study, we examined a new strategy to enhance collagen bundle formation and fibril orientation through administration of a collagen telopeptide binding peptide (CTBP). We found that CTBP, or a double-armed CTBP-PEG-CTBP derivative, could interact with both individual collagen molecules and fibrils. The CTBP interaction with collagen molecules inhibited fibril formation, whereas with reconstituted collagen fibrils, CTBP-PEG-CTBP promoted collagen bundle formation and fibril orientation. Finally, the addition of the CTBP derivative to the collagen matrix showed good cellular compatibility during in vitrocell culture. Taken together, our findings suggest that CTBP may have the potential for creating orderly, aligned, collagen-based matrices for tissue engineering.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Ligand binding, Protein folding, Thermal stability, Biochemistry