Title
A dual-color fluorescent biosensing platform based on WS2nanosheet for detection of Hg2+ and Ag+
Author
Xianwei Zuo, Huige Zhang, Qian Zhu, Weifeng Wang, Jie Feng, Xingguo Chen
Year
2016
Journal
Biosensors and Bioelectronics
Abstract
In this work, an effective dual-color fluorescent biosensing platform based on WS2nanosheets was developed for homogeneous detection of Hg2+ and Ag+. This sensing platform was constituted by exploiting the fluorescence quenching ability of WS2nanosheets and the interactions between WS2 nanosheets and DNA molecules. In the absence of additional any masking agents, the biosensor could achieve detection of Hg2+ and Ag+ in the same solution by monitoring fluorescence intensity changes at 525 nm and 583 nm, respectively. Hg2+ and Ag+ were selectively detected in the concentration range from 6.0–650.0 nM and 5.0–1000.0 nM, respectively, with the detection limit of 3.3 nM and 1.2 nM, respectively. It was also demonstrated that the WS2nanosheet-based sensing platform was suitable for the simultaneous detection of Hg2+and Ag+ in drinking water, serum and cell lysate samples. Moreover, the possible mechanism of fluorescence quenching by WS2 nanosheets was revealed to be related to static quenching, dynamic quenching, and Fo¨rster resonant energy transfer (FRET). This work extended the application of WS2 nanosheets to environmental monitoring and medical diagnosis.
Instrument
J-815
Keywords
Circular dichroism, DNA structure, Ligand binding, Materials, Nanostructures, Biochemistry