Title
A selective chemosensor for fluoride ion and its interaction with Calf Thymus DNA
Author
Soumen Ghosh, Abdulla Al Masum, Aniruddha Ganguly, Md. Maidul Islam, Md. Akhtarul Alam, Nikhil Guchhait
Year
2017
Journal
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Abstract
The amido-Schiff base 1 (N1, N3-bis (2-nitrobenzylidene)benzene-1,3-dicabohydrazide) containing a single bondCONHsingle bond group and single bondCHdouble bond; length as m-dashNsingle bond linkage has been synthesized by the condensation between isophthalic acid dihydrazide and o-nitrobenzaldehyde. This molecule can act as a fluoride ion sensor with high selectivity and sensitivity. Presence of nitro group in the phenyl ring may be responsible for the detection of fluoride ion visually with a dramatic color change from colorless to deep red in aqueous dimethyl sulphoxide solution. This Schiff base can be used as test kit for sensing of fluoride ion in the solid state. Compound 1 can detect fluoride also in commercially available toothpaste. As the compound has adequate solubility in DMSO–water mixture (7:93, v/v) and having some hydrogen bond donor and acceptor centers, we have investigated its nature of binding with Calf Thymus-DNA (CT-DNA) using theoretical molecular modelling and other experimental methods like UV–vis spectroscopy, circular dichroic and thermal melting studies. Thermodynamic parameters have been obtained using the well known Van't Hoff's equation. From both theoretical and experimental findings it has been observed that it can interact effectively with CT-DNA with binding energy − 7.55 kcal/mol to − 7.50 kcal/mol.
Instrument
J-815
Keywords
Circular dichroism, DNA structure, Ligand binding, Chemical stability, Biochemistry