Title
A two-photon tracer for human epidermal growth factor receptor-2: Detection of breast cancer in a live tissue
Author
Ji-Woo Choi, Seung Taek Hong, Dong Eun Kang, Kyu Cheol Paik, Man So Han, Chang Su Lim, Bong Rae Cho
Year
2016
Journal
Analytical Chemistry
Abstract
We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 over-expressing cells and breast cancers at a depth of 90−130 μm in live mouse tissue by TPM.
Instrument
J-715
Keywords
Circular dichroism, Secondary structure, Biochemistry