Title
Aberration in the structural paradigm of lens protein α crystallin by UV-C irradiation
Author
R. K. Ghosh, T. Kar, B. Dutta, A. Pathak, R. Rakshit, R. Basak, A. Das, K. Waheeda, P. Basak, M. Bhattacharyya
Year
2018
Journal
Applied Biological Chemistry
Abstract
The conformation of lens protein α crystallin was investigated using different spectroscopic techniques under normal and UV-C-irradiated condition. The structural elucidation of commercially available lens protein α crystallin under the effects of UV-C irradiation has never been reported earlier. To study the effects of irradiation on the lens protein, we used UV–visible spectroscopy, CD spectroscopy, and steady-state and time-resolved fluorescence measurements along with FTIR study, under increasing doses of UV-C irradiation. Using the secondary and tertiary structural changes as parameters for detecting conformational perturbation, we investigated the structural paradigm shift in the lens protein α crystallin. Increasing doses of UV-C radiation resulted in decreasing β sheet content of α crystallin from 30 to 10%. The fluorescence profile confirmed the formation of ROS species in the protein upon extensive exposure to UV-C irradiation. These results inferred UV-C irradiation may induce alteration of secondary structure of the lens protein leading to impaired biological functioning.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Biochemistry