Title
An aggregation induced emission enhancement-based ratiometric fluorescent sensor for detecting trace uranyl ion (UO22+) and the application in living cells imaging
Author
Xiaotong Chen, Lu Peng, Mengli Feng, Yu Xiang, Aijun Tong, Linfeng He, Bing Liu, Yaping Tang
Year
2017
Journal
Journal of Luminescence
Abstract
A ratiometric fluorescence detection method for trace amount of uranyl ion (UO22+) was developed in this study, based on aggregation induced emission enhancement (AIEE) characteristics of 3-hydroxyflavone salicylaldehyde azine (HFSA) and luminescent signal of UO22+-HFSA complex in soluble state. The fluorescent ratio (I457/I534) was linearly related to the concentration of UO22+ in the range of 0.5–20 ppb (part per billion). Analytical data showed that a detection limit of 0.5 ppb was achieved with the relative standard deviation (R.S.D.) 1.3% (n = 5). The proposed method could also be utilized for imaging UO22+ in living cells by ratiometric fluorescence changes.
Instrument
FP-8300
Keywords
Fluorescence, Quantitation, Aggregation, Sensors, Materials