Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin

July 28, 2017

Title

Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin

Author

Daniel Schneider, Tatjana Schneider, Joos Aschenbrenner, Franziska Mortensen, Martin Scheffner, Andreas Marx

Year

2016

Journal

Bioorganic and Medicinal Chemistry

Abstract

The Cu(I)-catalyzed alkyne–azide cycloaddition (CuAAC) has become increasingly important in the conjugation chemistry of biomolecules. For example, it is an efficient and convenient method to generate defined ubiquitin–protein conjugates. Here, we investigate the effect of surfactants on the efficiency of CuAAC for chemical protein ubiquitylation. We found that anionic surfactants enhance conjugate formation by up to 10-fold resulting in high yields even at low (i.e., micromolar) concentrations of the reactants. Notably, the herein investigated conjugates are functional and thus properly folded.

Instrument

J-815

Keywords

Circular dichroism, Protein folding, Medicinal, Biochemistry