Title
Antibacterial and Antibiofilm Activity and Mode of Action of Magainin 2 against Drug-Resistant Acinetobacter baumannii
Author
Min Kyung Kim, Nahee Kang, Su Jin Ko, Jonggwan Park, Eunji Park, Dong Won Shin, Seo Hyun Kim, Seung A. Lee, Ji In Lee, Seung Hyun Lee, Eun Gi Ha, Seung Hun Jeon, Yoonkyung Park
Year
2018
Journal
International Journal of Molecular Sciences
Abstract
Antimicrobial peptides (AMPs) are promising therapeutic agents for treating antibiotic-resistant bacterial infections. Previous studies showed that magainin 2 (isolated from African clawed fogs Xenopus laevis) has antimicrobial activity against gram-positive and gram-negative bacteria. The present study was conducted to investigate the antibacterial activity of magainin 2 against Acinetobacter baumannii. Magainin 2 showed excellent antibacterial activity against A. baumannii strains and high stability at physiological salt concentrations. This peptide was not cytotoxic towards HaCaT cells and showed no hemolytic activity. Biofilm inhibition and elimination were significantly induced in all A. baumannii strains exposed to magainin 2. We confirmed the mechanism of magainin 2 on the bacterial outer and inner membranes. Collectively, these results suggest that magainin 2 is an effective antimicrobial and antibiofilm agent against A. baumannii strains.
Full Article
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Vesicle interactions, Chemical stability, Biochemistry