Title
Antibacterial Natural Peptide Fractions from Indian Ganoderma lucidum
Author
Jigni Mishra, Rakhee Rajput, Kaushlesh Singh, Sanjeev Puri, Meenakshi Goyal, Anju Bansal, Kshipra Misra
Year
2017
Journal
International Journal of Peptide Research and Therapeutics
Abstract
Natural peptides are emerging as a leading alternative to conventional drugs and antibiotics, owing to their remarkable potency, better stability and less toxicity. Such peptides encompass numerous healing properties such as antimicrobial, anti-inflammatory, immunomodulatory, etc. Though plant- derived peptides have been widely studied for their therapeutic benefits, however, fungal peptides are still lesser explored. Ganoderma lucidum, a highly medicinal oriental mushroom comprises a vast array of phytoconstituents, namely flavonoids, phenolics, terpenoids, polysaccharides, proteins, glycolipids, etc and hence, is being used since several decades in traditional Chinese medicine (TCM) for its various ameliorative effects e.g. anti-inflammatory, antimicrobial, anti-proliferative and antioxidant properties. This study presents the isolation and characterization of antibacterial peptide fractions from fruiting body (GLF) and mycelium (GLM) of Indian G. lucidum. Representative amide bonds were identified in the fractions using established standard techniques. Peptide mass fingerprinting and HPLC confirmed the presence of cationic and hydrophobic amino acids in the peptide fractions which are known to be major structural features of antimicrobial peptides. Secondary structure prediction showed abundance of α-helices and random coils in GLF and GLM fractions respectively. The fractions exhibited appreciable antioxidant potential. Besides, these also possessed substantial antibacterial activity against Escherichia coli and Salmonella typhiwherein it was observed that generation of reactive oxygen species and induction of intracellular protein leakage within the bacterial cells were the possible mechanisms of inhibitory action.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Biochemistry