Anticancer Activity of Reconstituted Ribonuclease S-Decorated Artificial Viral Capsid
Yingbing Liang, Hiroto Furukawa, Dr. Kentarou Sakamoto, Dr. Hiroshi Inaba, Prof. Dr. Kazunori Matsuura
Ribonuclease S (RNase S) is an enzyme that exhibits anticancer activity by degrading RNAs within cancer cells; however, the cellular uptake efficiency is low due to its small molecular size. Here we generated RNase S-decorated artificial viral capsids with a size of 70–170 nm by self-assembly of the β-annulus-S-peptide followed by reconstitution with S-protein at neutral pH. The RNase S-decorated artificial viral capsids are efficiently taken up by HepG2 cells and exhibit higher RNA degradation activity in cells compared with RNase S alone. Cell viability assays revealed that RNase S-decorated capsids have high anticancer activity comparable to that of standard anticancer drugs.
Ribonuclease, anticancer, drug