Antiinflammation constituents from Curcuma zedoaroides
Pattreeya Tungcharoen, Chatchai Wattanapiromsakul, Pimpimon Tansakul, Seikou Nakamura, Hisashi Matsuda, Supinya Tewtrakul
Curcuma zedoaroides, a Zingiberaceae species, has been used for snake bite antidote and wound care in Thailand. Seven compounds were isolated from the bioactive chloroform extract consisted of one new guaiane sesquiterpene lactone, 5‐epiphaeocaulisin A (4) and one new diarylheptanoid, 1,2,3,5‐tetrahydroxy‐1‐(4‐hydroxy‐3‐methoxyphenyl)‐7‐(4‐hydroxyphenyl) heptane (7), together with five known guaiane‐type sesquiterpene lactones including gweicurculactone (1), zedoalactone B (2), phaeocaulisin C (3), zedoalactone H (5), and zedoalactone E (6). The antiinflammation was investigated on NO and TNF‐α production using RAW264.7 cells. In addition, the expressions of genes involved in inflammation including iNOS, COX‐2, and TNF‐α were assessed. The results found that Compounds 1–7 presented their antiinflammation against NO production. The most potential effects were demonstrated on Compounds 1 and 7 with IC50 of 27.3 and 32.6 μM, respectively. Although, Compounds 1 and 7 did not inhibit TNF‐α production, their suppression on iNOS and COX‐2 mRNA expression were revealed. These results support the ability of chloroform fraction, Compounds 1 and 7 on antiinflammation, whereas others exhibited moderate and mild effect.
Circular dichroism, Absolute configuration, Pharmaceutical