Title
AS1411 derivatives as carriers of G-quadruplex ligands for cervical cancer cells
Author
Joana Figueiredo, Jéssica Lopes-Nunes, Josué Carvalho, Francisca Antunes, Márcia Ribeiro, Maria Paula Cabral Campello, António Paulo, Artur Paiva, Gilmar F. Salgado, João A. Queiroz, Jean-Louis Mergny, Carla Cruz
Year
2019
Journal
International Journal of Pharmaceutics
Abstract
Nucleic acid aptamers can specifically bind to target molecules on the cell membrane that mediate their entrance into the cells. Their small size, high binding affinity, specificity, good biocompatibility, stability and low immunogenicity make them ideal drug delivery systems for cancer therapy. These biopharmaceuticals have potential for the delivery of anticancer compounds to diseased tissues, increasing their effectiveness while mitigating the off-target toxicity towards healthy cells. Herein, we have studied two quadruplex-forming DNA sequences derived from the nucleolin-targeted aptamer AS1411 as supramolecular carriers for the cancer-selective delivery of acridine orange derivatives (C3, C5 and C8) in cervical cancer cells.
Instrument
J-815
Keywords
Circular dichroism, G-quadruplex structure, Ligand binding, Thermal stability, Biochemistry