Assessment of intercalative interaction of the benzophenanthridine plant alkaloid nitidine with higher ordered forms of RNA: Spectroscopic evaluation
Lucy Haque, Sutanwi Bhuiya, Suman Das
New Journal of Chemistry
Spectrophotometric, spectropolarimetric, viscometric and spectrofluoimetric analysis of the binding of the alkaloid nitidine to the double and triple helical forms of RNA have served to enlighten the ability of the drug to produce changes in the RNA structure. The experimental data assembled here convey that nitidine is able to intercalate into the base pairs of poly(A).poly(U) and poly(U).poly(A)*poly(U) in the specified conditions and the strength of interaction was in the order of 106 M-1 as calculated from UV-absorption study. Binding constants showed that NIT binds more strongly with the triple helical forms of RNA rather than the corresponding parent duplex.
Circular dichroism, RNA structure, Ligand binding, Induced circular dichroism, Polymers, Biochemistry