Title
Backbone engineering within a latent β-hairpin structure to design inhibitors of polyglutamine amyloid formation
Author
Karunakar Kar, Matthew A. Baker, George A. Lengyel, Cody L. Hoop, Ravindra Kodali, In-Ja Byeon, W. Seth Horne, Patrick C.A. van der Wel, Ronald Wetzel
Year
2016
Journal
Journal of Molecular Biology
Abstract
Candidates for the toxic molecular species in the expanded polyglutamine (polyQ) repeat diseases range from various types of aggregates to “misfolded” monomers. One way to vet these candidates is to develop mutants that restrict conformational landscapes. Previously we inserted two self-complementary β-hairpin enhancing motifs into a short polyQ sequence to generate a mutant, here called “βHP”, that exhibits greatly improved amyloid nucleation without measurably enhancing β-structure in the monomer ensemble. We extend these studies here by introducing single backbone H-bond impairing modifications αN-methyl Gln or l-Pro at key positions within βHP. Modifications predicted to allow formation of a fully H-bonded β-hairpin at the fibril edge while interfering with H-bonding to the next incoming monomer exhibit poor amyloid formation and act as potent inhibitors in trans of simple polyQ peptide aggregation. In contrast, a modification that disrupts intra-β-hairpin H-bonding within βHP, while also aggregating poorly, is ineffective at inhibiting amyloid formation in trans. The inhibitors constitute a dynamic version of the edge-protection negative design strategy used in protein evolution to limit unwanted protein aggregation. Our data support a model in which polyQ peptides containing strong β-hairpin encouraging motifs only rarely form β-hairpin conformations in the monomer ensemble, but nonetheless take on such conformations at key steps during amyloid formation. The results provide insights into polyQ solution structure and fibril formation while also suggesting an approach to the design of inhibitors of polyQ amyloid growth that focuses on conformational requirements for fibril and nucleus elongation.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Biochemistry