Title
Binding of ethyl pyruvate to bovine serum albumin: Calorimetric, spectroscopic and molecular docking studies
Author
Mallika Pathak, Rashmi Mishra, Paban K. Agarwala, Himanshu Ojha, Bhawna Singh, Anju Singh, Shrikant Kukreti
Year
2016
Journal
Thermochimica Acta
Abstract
Various in vitro and in vivo studies have shown the anti-inflammatory and anticancer potential role of ethyl pyruvate. Bio-distribution of drugs is significantly influenced by the drug-serum protein binding. Therefore, the binding mechanism of the ethyl pyruvate with bovine serum albumin was investigated using UV–vis absorption, fluorescence, circular dichroism, isothermal titration calorimetry and molecular docking techniques. Absorption and fluorescence quenching studies indicated the binding of ethyl pyruvate with protein. Circular dichroism spectra of bovine serum albumin confirmed significant change in the conformation of protein upon binding. Thermodynamic data confirmed that ethyl pyruvate binds to bovine serum albumin at the two different sites with high affinity. Binding of ethyl pyruvate to bovine serum albumin involves hydrogen bonding, van der Waal and hydrophobic interactions. Further, docking studies indicated that ethyl pyruvate could bind significantly at the three binding sites. The results will definitely contribute to the development of ethyl pyruvate as drug.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Protein folding, Ligand binding, Biochemistry