Title
CasuL: A new lectin isolated from Calliandra surinamensis leaf pinnulae with cytotoxicity to cancer cells, antimicrobial activity and antibiofilm effect
Author
Thamara Figueiredo Procópio, Leydianne Leite de Siqueir Patriota, Maiara Celine de Moura, Pollyanna Michelle da Silva, Ana Patrícia Silva de Oliveira, Lidiane Vasconcelos do Nascimento Carvalho, Thâmarah de Albuquerque Lima, Tatiana Soares, Túlio Diego da Silva, Luana Cassandra Breitenbach Barroso Coelho, Maira Galdino da Rocha Pitta, Moacyr Jesus Barreto de Melo Rêgo, Regina Celia Bressan Queiroz de Figueiredo, Patrícia Maria Guedes Paiva, Thiago Henrique Napoleão
Year
2017
Journal
International Journal of Biological Macromolecules
Abstract
This work describes the isolation of a lectin (CasuL) from the leaf pinnulae of Calliandra surinamensis and the evaluation of its cytotoxic, antimicrobial and antibiofilm properties. Proteins from pinnulae extract were precipitated with ammonium sulphate (60% saturation) and submitted to Sephadex G-75 chromatography, which yielded isolated CasuL (purification factor: 113). Native CasuL is an acidic protein (pI 5.82) with a relative molecular mass of 48 kDa. This lectin is also an oligomeric protein composed of three subunits and mass spectrometry revealed similarities with a Sorghum bicolor protein. CasuL did not undergo unfolding when heated but changes in conformation and hemagglutinating activity were detected at basic pH. CasuL did not reduce the viability of human peripheral blood mononuclear cells but was toxic to leukemic K562 cells (IC5067.04 ± 5.78 μg/mL) and breast cancer T47D cells (IC50: 58.75 ± 2.5 μg/ mL). CasuL (6.25–800 μg/mL) only showed bacteriostatic effect but was able to reduce biofilm formation by Staphylococcus saprophyticcus and Staphylococcus aureus (non-resistant and oxacillin-resistant isolates). CasuL showed antifungal activity against Candida krusei causing alterations in cell morphology and damage to cell wall. In conclusion, the pinnulae of C. surinamensis leaves contain a thermo-stable lectin with biotechnological potential as cytotoxic, antibiofilm, and antifungal agent.
Instrument
FP-6300
Keywords
Fluorescence, Protein structure, Thermal stability, Chemical stability, Natural products, Biochemistry