Title
Characterization and analysis of binding of Thioflavin T with partially folded and native states of α–lactalbumin protein by calorimetric and spectroscopic techniques
Author
Moumita Dasgupta, Nand Kishore
Year
2017
Journal
International Journal of Biological Macromolecules
Abstract
We have analysed binding of Thioflavin T (ThT) with molten globule (MG) and native (N) states of α–lactalbumin (α–LA) by using calorimetry and spectroscopy. ThT has been widely used for detection of amyloid fibrils from enhancement of its fluorescence emission intensity. Instead of the spectral changes of ThT, we, rather, monitored the changes occurring in the spectral properties of the MG and N states upon interaction with ThT, from fluorescence, absorbance and circular dichroism (CD) spectroscopy. Our novel and most important observation is non-fluorescent mode of binding of ThT to the MG state of α-LA suggesting that the mechanism of binding is distinctly different from that of association with the protein fibrils. CD and DLS (Dynamic Light Scattering) results show the absence of any major structural and size changes in the protein upon ThT binding. The thermodynamic parameters of binding of ThT with the MG and N states of α–LA obtained from Isothermal Titration Calorimetry (ITC) experiments show the formation of stable complex between ThT and α–LA (both MG and N states) and also provide insights on the probable mode of interaction of ThT with the MG and N states of α–LA.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Tertiary structure, Ligand binding, Chemical stability, Biochemistry