Title
Chemical Constituents of the Rare Cliff Plant Oresitrophe rupifraga and Their Anti-neuroinflammatory Activity
Author
Xi-Ying Wu, Juan Xiong, Xin-Hua Liu, Jin-Feng Hu
Year
2016
Journal
Chemistry and Biodiversity
Abstract
Four new (1−4) and thirteen known (5−17) compounds were isolated from a rare cliff plant, Oresitrophe rupifraga. Based on spectroscopic evidence, the new structures were established to be [(2S,3R,4R)-4-(4-methoxybenzyl)-2-(4-methoxyphenyl)tetrahydrofuran-3-yl]methanol (1), (3α)-23-(acetyloxy)-3-hydroxyolean-12-en-29-oic acid (2), 3α,23-isopropylidenedioxyolean-12-en-29-oic acid (3, artifact of isolation), and (3β,15β)-3-hydroxycholest-5-en-15-yl β-d-glucopyranoside (4), respectively. Among the isolates, compounds 1, 4, epieudesmin (7), and 1-O-(9Z,12Z,15Z-octadecatrienoate)glycerol (17) were found to show significant anti-neuroinflammatory effects by inhibiting the nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated murine BV-2 microglial cells, with IC50 values of 7.21, 9.39, 4.96, and 8.51μm, respectively.
Instrument
J-810
Keywords
Circular dichroism, Absolute configuration, Pharmaceutical