Title
Chiral Restructuring of Peptide Enantiomers on Gold Nanomaterials
Author
Joseph M. Slocik, Patrick B. Dennis, Alexander O. Govorov, Nicholas M. Bedford, Yang Ren, Rajesh R. Naik
Year
2019
Journal
ACS Biomaterials Science & Engineering
Abstract
The use of biomolecules has been invaluable at generating and controlling optical chirality in nanomaterials; however, the structure and properties of the chiral biotemplate are not well understood due to the complexity of peptide–nanoparticle interactions. In this study, we show that the complex interactions between d-peptides and gold nanomaterials led to a chiral restructuring of peptides as demonstrated by circular dichroism and proteolytic cleavage of d-peptides via gold-mediated inversion of peptide chirality. The gold nanoparticles synthesized using d-peptide produce a highly ordered atomic surface and restructured peptide bonds for enzyme cleavage. Differences in gold nanoparticle catalyzed reduction of 4-nitrophenol were observed on the basis of the chiral peptide used in nanoparticle synthesis. Notably, the proteolytic cleavage of d-peptides on gold provides an opportunity for designing nanoparticle based therapeutics to treat peptide venoms, access new chemistries, or modulate the catalytic activity of nanomaterials.
Instrument
J-815
Keywords
Circular dichroism, Stereochemistry, Secondary structure, Nanostructures, Ligand binding, Biochemistry, Materials