Title
Circular dichroism spectroscopic investigation of double-decker phthalocyanine with G-Quadruplex as promising telomerase inhibitor
Author
Efkan Bağda, Esra Bağda, Ebru Yabaş
Year
2016
Journal
Journal of Molecular Structure
Abstract
In the present study, interaction of a double-decker phthalocyanine with two G-quadruplex DNA, Tel 21 and cMYC, was investigated. To the best of our knowledge, this is the first study about G-quadruplex-double decker phthalocyanine interaction. The spectrophotometric titration method was used for binding constant calculations. From the binding constants, it can be said that double-decker phthalocyanine more likely to bind Tel 21 rather than cMYC. The conformational changes upon binding were monitored via circular dichroism spectroscopy. The ethidium bromide replacement assay was monitored spectrofluorometrically.
Instrument
J-815
Keywords
Circular dichroism, DNA structure, Ligand binding, Biochemistry