Title
Complicated function of dopamine in Aβ-related neurotoxicity: Dual interactions with Tyr10 and SNK(26–28) of Aβ
Author
Mengmeng Liu, Lu Kou, Yannan Bin, Liping Wan, Juan Xiang
Year
2016
Journal
Journal of Inorganic Biochemistry
Abstract
With the capability to inhibit the formation of amyloid β peptides (Aβ) fibril, dopamine (DA) and other catechol derivatives have been considered for the potential treatment of Alzheimer's disease (AD). Such treatment, however, remains debatable because of the diverse functions of Aβ and DA in AD pathology. Moreover, the complicated oxidation accompanying DA has caused the majority of the previous research to focus on the binding of DA oxides onto Aβ. The molecular mechanism by which Aβ interacts with the reduction state of DA, which is correlative with the brain function, should be urgently explored. By controlling rigorous anaerobic experimental conditions, this work investigated the molecular mechanism of the Aβ/DA interaction, and two binding sites were revealed. For the binding of DA, Tyrosine (Tyr10) was identified as the strong binding site, and serine-asparagine-lysing (SNK(26–28)) segment was the weak binding segment. Furthermore, the Thioflavin T (THT) fluorescence confirmed DA's positive function of inhibiting Aβ aggregation through its weakly binding with SNK(26–28) segment. Meanwhile, 7-OHCCA fluorescence exhibited DA's negative function of enhancing radical dotOH generation through inhibiting the Aβ/Cu2 + coordination. The viability tests of the neuroblastoma SH-SY5Y cells displayed that the coexistence of DA, Cu2 +, and Aβ induced lower cell viability than free Cu2 +, indicating the significant negative effect of excessive DA on AD progression. This research revealed the potential DA-induced damage in AD brain, which is significant for understanding the function of DA in AD neuropathology and for designing a DA-related therapeutic strategy for AD.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Biochemistry