Title
Construction of Ribonuclease-Decorated Artificial Virus-like Capsid by Peptide Self-assembly
Author
Kazunori Matsuura, Junpei Ota, Seiya Fujita, Yuriko Shiomi, Hiroshi Inaba
Year
2020
Journal
Journal of Organic Chemistry
Abstract
Artificial virus-like capsids decorated with ribonuclease S (RNase S) on their exterior were constructed by the self-assembly of β-annulus-S-peptide and the interaction between S-peptide moiety and S-protein. The β-annulus-S-peptide was synthesized by native chemical ligation of β-annulus-SBz peptide with Cys-containing S-peptide that self-assembled into artificial virus-like capsids of approximately 47 nm in size. Reconstruction of RNase S on the artificial virus-like capsids afforded spherical assembly attached small spheres on the surface, which retained ribonuclease activity.
Full Article
Instrument
FP-8200, J-820
Keywords
Circular dichroism, DNA binding, Secondary structure, Protein structure, Chemical stability, Biochemistry, Organic chemistry