Title
Controlled enzymatic stability and release characteristics of supramolecular chiral peptide amphiphile nanofiber gels
Author
Aygul Zengin, Goksu Cinar, Mustafa O. Guler
Year
2017
Journal
Current Applied Physics
Abstract
Supramolecular bioarchitectures formed by assembly of achiral or chiral building blocks play important roles in various biochemical processes. Stereochemistry of amino acids is important for structural organization of peptide and protein assemblies and structure-microenvironment interactions. In this study, oppositely charged peptide amphiphile (PA) molecules with L-, D- and mixture of l- and d-amino acid conformations are coassembled into supramolecular nanofibers and formed self-supporting gels at pH 7.4 in water. The enzymatic stability of the PA nanofiber gels was studied in the presence of proteinase K enzyme, which digest a broad spectrum of proteins and peptides. The structural changes on the chiral PA nanofibers were also analyzed at different time periods in the presence of enzymatic activity. Controlled release of a model cargo molecule through the chiral PA nanofiber gels was monitored. The diffusivity parameters were measured for all gel systems. Release characteristics and the enzymatic stability of the peptide nanofiber gels were modulated depending on organization of the chiral PA molecules within the supramolecular assemblies.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Nanostructures, Stereochemistry, Biochemistry, Materials