Title
Crowding and conformation interplay on human DNA G-quadruplex by ultraviolet resonant Raman scattering
Author
Silvia Di Fonzo, Cettina Bottari, John W. Brady, Letizia Tavagnacco, Marco Caterino, Luigi Petraccone, Jussara Amato, Concetta Giancola, Attilio Cesàro
Year
2019
Journal
Physical Chemistry Chemical Physics
Abstract
The G-quadruplex-forming telomeric sequence (TTAGGG)4TT was investigated by polarized Ultraviolet Resonance Raman Scattering (UVRR) at 266 nm. The presence of 40% poly(ethylene glycol) and the so-called “self-crowding” condition were used to induce the hybrid-to-parallel topology transition. Analysis of frequency shifts with temperature showed the role of several functional groups in the topological transitions and provides structural dynamical information. Circular dichroism under similar conditions was used as a reference. UVRR shed light on the effect of intramolecular interactions and of local and environmental dynamics in promoting different G-quadruplex topologies, induced by solution conditions or by temperature changes. Overall, these findings showed the enormous potential of this spectroscopy for G-quadruplex conformational studies.
Instrument
J-815
Keywords
Circular dichroism, DNA structure, Thermal stability, Thermodynamics, Biochemistry