Development and Validation of Rapid HPLC Method for Determination of Doxofylline in Bulk Drug and Pharmaceutical Dosage Forms
Ashu Mittal and Shikha Parmar
Journal of Analytical Chemistry
A simple, selective, rapid, and economical reversed phase high performance liquid chromatogra phy(RPHPLC) method for the determination of doxofylline in the commercial dosage form has been devel oped and validated. The separation and quantification were achieved on an HiQ Sil C 18 W column using a mobile phase of acetonitrile : buffer (50 : 50), pH 3, at a flow rate of 1 mL/min with detection of analyte at 272 nm. The separation was achieved within 3.1 ± 0.3 min for doxofylline sample. The method showed good linearity in the range of 10–80 µg/mL. The intra and inter day RSD ranged from 0.37–0.53%. The recovery (mean ± S.D.) of low, middle and high concentrations were 100.04 ± 0.80, 100.01 ± 0.20, 100.07 ± 0.30 respectively. Limit of detection and limit of quantification were 0.03 and 0.1 µg/mL, respectively.
HPLC, Pharmaceutical, Doxofylline, Dosage