Title
Diterpenoids with inhibitory activity of nitrite production from Croton floribundus
Author
Suzana Aparecida S. Queiroz, Meri Emili F. Pinto. Antonio F. Bobey, Helena M. Russo, Andrea N. L. Batista, Joao M. Batista Jr., Ana C. Codo, Alexandra I. Medeiros, Vanderlan S. Bolzani
Year
2019
Journal
Journal of Ethnopharmacology
Abstract
Croton floribundus Spreng. (Euphorbiaceae), popularly known as Capixinguí, stands out due to its widespread use in traditional medicine to treat wounds, syphilis, hemorrhoids, eye diseases and as a purgative. To characterize clerodanes diterpenes from C. floribundus and to evaluate the effects of the fraction and diterpenes (1–5) on inhibition of nitrite production. The hydroethanolic root extract of C. floribundus was fractionated on a solid phase extraction column to obtain the fraction named Fr80%. From this, five compounds were obtained and characterized. The absolute configuration of compound 1 was determined by a combination of electronic and vibrational circular dichroism spectroscopies. Additionally, compounds 1–5 were evaluated for their inhibitory effects on nitrite production induced by lipopolysaccharide (LPS) in RAW 264 macrophage cell. Five clerodane diterpenoids were characterized, and the absolute stereochemistry of 1 was established as 3R,4R,5R,8R,9R,10S,12S. The IC50 values obtained through inhibition of nitrite production were 28.52 ± 2.21 μM (1), 40.26 ± 2.79 μM (2), 25.47 ± 2.16 μM (3), 35.78 ± 2.93 μM (4) and 40.58 ± 4.78 μM (5). In the tested concentrations, the samples presented low toxicity in macrophages. Four new diterpenes were characterized from C. floribundus, these being croflorins A-D (1–4) and a known halimane (5). These compounds exhibited inhibitory effect on nitrite production.
Instrument
J-815
Keywords
Circular dichroism, Absolute configuration, Medicinal, Pharmaceutical