Title
Effects of structurally stabilized EGF and bFGF on wound healing in type I and type II diabetic mice
Author
Seong Mi Choi, Kyoung-Mi Lee, Hyun Jung Kim, Ik Kyu Park, Hwi Ju Kang, Hang-Cheol Shin, Dawoon Baek, Yoorim Choi, Kwang Hwan Park, Jin Woo Lee
Year
2018
Journal
Acta Biomaterialia
Abstract
Diabetes mellitus comprises a multiple metabolic disorder that affects millions of people worldwide and consequentially poses challenges for clinical treatment. Among the various complications, diabetic ulcer constitutes the most prevalent associated disorder and leads to delayed wound healing. To enhance wound healing capacity, we developed structurally stabilized epidermal growth factor (ST-EGF) and basic fibroblast growth factor (ST-bFGF) to overcome limitations of commercially available EGF (CA-EGF) and bFGF (CA-bFGF), such as short half-life and loss of activity after loading onto a matrix. Neither ST-EGF nor ST-bFGF was toxic, and both were more stable at higher temperatures than CA-EGF and CA-bFGF. We loaded ST-EGF and ST-bFGF onto a hyaluronate-collagen dressing (HCD) matrix, a biocompatible carrier, and tested the effectiveness of this system in promoting wound healing in a mouse model of diabetes. Wounds treated with HCD matrix loaded with 0.3 μg/cm2 ST-EGF or 1 μg/cm2 ST-bFGF showed a more rapid rate of tissue repair as compared to the control in type I and II diabetes models. Our results indicate that an HDC matrix loaded with 0.3 μg/cm2 ST-EGF or 1 μg/cm2 ST-bFGF can promote wound healing in diabetic ulcers and are suitable for use in wound dressings owing to their stability for long periods at room temperature.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Thermal stability, Thermodynamics, Biochemistry, Medicinal