Title
Enantioselectivity of D-amino acid oxidase in the presence of ionic liquids
Author
Qingju Liu, Chuanfang Zhao, Jincheng Huang, Li Chen, Kunhao Yang, Lingling Gong, Yuguo Du, Chuyi Yu, Li Wu, Xiangjun Li, Yujian He
Year
2017
Journal
RSC Advances
Abstract
In this paper, enantioselectivities of D-amino acid oxidase (DAAO) in ten ionic liquids were investigated in detail. Among the ionic liquids studied, 3-methylimidazolium formate ([MIM][COO]) was able to trigger enzyme activation with L-Ala as substrate. The enzyme activity of DAAO reached a maximum of 4.26 U mg−1 min−1 in the presence of 40% [MIM][COO], while no activity toward L-Ala was observed when DAAO was incubated with other ILs. Moreover, DAAO activities towards other L-amino acids such as L-Pro and L-Arg were also improved in [MIM][COO], which indicated that the enzymatic enantioselectivity of amino acids was lowered. This was further confirmed by capillary electrophoresis, circular dichrosim and fluorescence analysis. Molecular optimization supported that the protein–ionic liquid interactions modulated the structure of the enzyme, especially in [MIM][COO], leading to relaxation in the enantioselectivity of DAAO. This observation and exploration might open a new window for modulation enantioselectivity resolution in ionic liquids.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Chemical stability, Biochemistry